dacF [2020-05-19 16:08:37]

penicillin-binding protein I, D-alanyl-D-alanine carboxypeptidase

locus: BSU_23480

pI: 9.91

mw: 43.13 kDa

protein length: 389 aa

gene length: 1170 bp

function: carboxypeptidase

product: penicillin-binding protein I, D-alanyl-D-alanine carboxypeptidase

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.7|Penicillin-binding proteins]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 2,445,094 → 2,446,263

The protein

Catalyzed reaction/ biological activity

• modifies degree of cross-linking of glycan strands in peptidoglycan
• Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine (according to UniProt)

Protein family

• [SW|Peptidase S11 family] (according to UniProt)

Paralogous protein(s)

• [protein|7C3081BC8D416127A881627EB56C0628359111CF|DacA], [protein|76825D77907E8CE47B17ED56D7E2A348B1ADA5EC|DacB]

Structure

• [PDB|4K91] (from Pseudomonas aeruginosa, 38% identity) [pubmed|23629710]

[SW|Localization]

• secreted (via signal peptide) (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|F7AD78F9AB98A150E8CDFC1D01A9F938FF9F8BD1|dacF]-[gene|3936E91C062074BFE4284B54A8CC33F35F94F5EE|spoIIAA]-[gene|C8B867758330355F44C63CC284DC7AA3061FB9F1|spoIIAB]-[gene|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|15699190], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]
  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  regulation

  • ''[SW|spoIIAA]'': expressed early during sporulation
  • strongly repressed in the presence of salt (1.2 M NaCl) [pubmed|32419322]

Biological materials

Mutant

• BKE23480 (Δ[gene|F7AD78F9AB98A150E8CDFC1D01A9F938FF9F8BD1|dacF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCAAAAGCCCTCCATT, downstream forward: _UP4_TAATTATGCCGAATGACCAC
• BKK23480 (Δ[gene|F7AD78F9AB98A150E8CDFC1D01A9F938FF9F8BD1|dacF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCAAAAGCCCTCCATT, downstream forward: _UP4_TAATTATGCCGAATGACCAC

References

Reviews

• 18266855

Original publications

• 16497325,8936302,9864321,12107147,20817675,22123250,23629710